Detailed Notes on cytometer
Detailed Notes on cytometer
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Details-enriched characterization of cell populations. Morphological data from photos can incorporate on the richness of flow cytometry details. By way of example, the determine demonstrates an usually regular apoptosis assay using Annexin V and PI, adding mobile imaging to characterize cells in Every single population to reveal morphologically distinctive capabilities. These insights could not are already received from multiplex staining by itself.
As samples are obtained on the Attune CytPix Flow Cytometer, the higher-speed brightfield camera captures and outlets pictures of detected activities at a level up to six,000 pictures for every 2nd, with regards to the flow price and picture dimension. For increased flexibility, Attune Cytometric Program helps you to modify the picture capture frequency as necessary and choose a particular gate for image capture, if expected.
Blend acoustic concentrating technologies with substantial-parameter Examination for adaptable spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating by using a large-velocity brightfield digicam for simultaneous significant throughput flow cytometry and significant resolution brightfield imaging.
"A picture is not very well worth 1000 dots, however it seriously really helps to see photographs. It is excellent to find out pictures that correlate with staining. Such as, AnnexinV-positive cells that are morphologically various than healthy cells, or CD14+ monocytes which can be much larger and a lot more textured than CD3+/CD4+/CD14- lymphocytes.
Also, it can be illuminating to determine the amount of doublets slip by way of classic singlet gates, and good to verify the position of unpredicted double-constructive occasions in my Investigation."
Pair your Attune flow cytometer using an autosampler for elevated workflow efficiency, and include robotic integration for max throughput.
Dependable image high quality even at superior throughput Acoustic focusing as well as a large-velocity camera Merge to impression these T cells consistently at small or high flow fees. Quickly change aim and digital camera configurations to fulfill experimental prerequisites.
The Attune NxT cytometer is definitely the trusted workhorse instrument your lab can depend on for dependable and trusted effectiveness. The Attune CytPix model features a higher-speed brightfield digicam and AI-pushed impression analysis software, with current updates such as device learning applications and an intuitive user interface, enabling Increased information precision, precision, and a lot quicker insights into sample biology.
Potentially most importantly, these functions comprise cells of Evidently various phenotypes which could lead on to incorrect conclusions relating to double good functions (specifically in rare populations).
AI-pushed automatic image Examination Together with the Attune read more Cytometric Program interprets celebration features into unique morphology parameters which can be combined with standard fluorescence and scatter parameters to expose really reproducible population changes and distinctive subsets.
Software Notice: Automatic impression Investigation cuts down user-to-user variability in flow cytometry gating techniques
Constructed-in dimensionality reduction tools improved resolution into populations. Fluorescently gated populations back again-gated onto UMAP plot to confirm envisioned populace resolution in lysed complete human blood. Consumer can view events from Each individual cluster for visual confirmation of mobile phenotypes.
Flexible payment plan from Agilent Monetary Options enable you to have the essential instrumentation you'll need for instant undertaking, or to increase your lab.
At the guts of each Attune Flow Cytometer lies our innovative acoustic concentrating technological innovation, which utilizes seem waves to meticulously align cells inside the flow cell. This modern method surpasses standard hydrodynamic approaches, leading to more rapidly processing speeds, Excellent knowledge top quality, and elevated sample throughput.
As the mobile line experienced Formerly been analyzed over the Attune CytPix Flow Cytometer, the researchers went again to the photographs and had been in the position to document the microbial an infection at least five days earlier.
Agilent Price Promise assures you not less than a decade of instrument use through the day you purchase. Or else, We'll credit history you with the residual worth of the technique toward an upgraded design.
What appeared to be a homogeneous population is in fact far more assorted—an insight that should be regarded when interpreting outcomes.